rpmjResearch'n Practical Medicine JournalResearch and Practical Medicine Journal2410-1893"QUASAR", LLC10.17709/2409-2231-2020-7-4-4rpmj-631Research ArticleОригинальные статьи. Онкология, лучевая терапияOriginal Articles. ОncologyКачественная и количественная характеристика разных типов опухолевых микрососудов в зависимости от гистологического типа рака шейки маткиQualitative and quantitative characteristics of different types of tumor microvessels depending on the histological type of cervical cancerhttps://orcid.org/0000-0001-8371-740XСеньчуковаМ. А.SenchukovaM. A.

Сеньчукова Марина Алексеевна – д.м.н., профессор кафедры лучевой диагностики, лучевой терапии, онкологии, врач-онколог хирургического торакального отделения

460000, г. Оренбург, ул. Советская, д. 6

460021, г. Оренбург, просп. Гагарина, д. 11 

SPIN: 9877-8828

AuthorID: 583884

Scopus Author ID: 56084387100

ResearcherID: W-6584-2018

Marina A. Senchukova – Dr. Sci. (Med.), professor of the radiation diagnostics, radiation therapy, oncology department, physician -oncologist of the surgical thoracic department

6 Sovetskaya str., Orenburg, 460000

11 Gagarin ave., Orenburg, 460021

SPIN: 9877-8828

AuthorID: 583884

Scopus Author ID: 56084387100

ResearcherID: W-6584-2018

https://orcid.org/0000-0002-5796-3719МакароваЕ. В.MakarovaE. V.

Макарова Елена Викторовна – аспирант кафедры лучевой диагностики, лучевой терапии, онкологии, врач отделения ультразвуковой диагностики 

460000, г. Оренбург, ул. Советская, д. 6

460021, г. Оренбург, просп. Гагарина, д. 11 

Elena V. Makarova – PhD student of the radiation diagnostics, radiation therapy, oncology department, doctor of the department of ultrasound

6 Sovetskaya str., Orenburg, 460000

11 Gagarin ave., Orenburg, 460021

malena2419@yandex.ruhttps://orcid.org/0000-0002-0571-2803ШурыгинаЕ. И.ShuryginaE. I.

Шурыгина Елена Ивановна – ассистент кафедры патологической анатомии, врач патологоанатомического отделения

460000, г. Оренбург, ул. Советская, д. 6

460021, г. Оренбург, просп. Гагарина, д. 11 

Elena I. Shurygina – department of pathology, physician at the department of pathology

6 Sovetskaya str., Orenburg, 460000

11 Gagarin ave., Orenburg, 460021

https://orcid.org/0000-0002-4873-4455ВолченкоН. Н.VolchenkoN. N.

Волченко Надежда Николаевна – д.м.н., профессор, заведующая отделом онкоморфологии

125284, г. Москва, 2-й Боткинский проезд, д. 3

AuthorID: 290657

Nadezhda N. Volchenko – Dr. Sci. (Med.), professor, head of the tumor morphology department

3 2nd Botkinskiy travel, Moscow, 125284

AuthorID: 290657

ФГБОУ ВО «Оренбургский государственный медицинский университет» Министерства здравоохранения Российской Федерации; ГБУЗ «Оренбургский областной клинический онкологический диспансер»РоссияOrenburg State Medical University; Orenburg Regional Clinical Oncology DispensaryRussian FederationМНИОИ им. П.А.Герцена – филиал ФГБУ «Национальный медицинский исследовательский центр радиологии» Министерства здравоохранения Российской ФедерацииРоссияP.A.Hertsen Moscow Oncology Research Institute – Branch of the National Medical Research Radiological CentreRussian Federation202021122020743650Copyright © Сеньчукова М.А., Макарова Е.В., Шурыгина Е.И., Волченко Н.Н., 20202020Сеньчукова М.А., Макарова Е.В., Шурыгина Е.И., Волченко Н.Н.Senchukova M.A., Makarova E.V., Shurygina E.I., Volchenko N.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.rpmj.ru/rpmj/article/view/631Цель исследования

Цель исследования. Дать качественную и количественную оценку разным типам опухолевых микрососудов в зависимости от гистологического типа рака шейки матки (РШМ).

Материал и методы

Материал и методы. Объектом исследования служили образцы опухоли 76 пациенток с РШМ I–IIА стадии, получивших первым этапом хирургическое лечение. Иммуногистохимическое исследование проведено с использованием антител к CD34 и подопланину. Статистическую обработку результатов исследования выполняли с использованием программы Statistica 6.0.

Результаты

Результаты. При РШМ выделены следующие типы опухолевых микрососудов: обычные микрососуды, дилятированные капилляры (ДК) c обычной эндотелиальной выстилкой, ДК со слабо выраженной экспрессией CD34, ДК контактного типа, капилляры в солидном компоненте опухоли, атипичные дилятированные капилляры (АДК), структуры с частичной эндотелиальной выстилкой, лимфатические капилляры в лимфоидных и полиморфноклеточных инфильтратах. Установлено, что с прогнозом заболевания при раннем РШМ коррелировали ДК со слабовыраженной экспрессией CD34 (р=0,0005), ДК контактного типа (р=0,000005) и капилляры в солидном компоненте опухоли (р=0,006). Частота выявления этих типов опухолевых микрососудов, была ниже при прогностически благоприятном плоскоклеточном неороговевающем РШМ, тогда как для прогностически неблагоприятных плоскоклеточном ороговевающем РШМ и аденокарциноме шейки матки были характерны более высокие значения ДК со слабовыраженной экспрессией CD34 (р=0,004 и р=0,005, соответственно) и ДК контактного типа (р=0,02 и р=0,14, соответственно).

Purpose of the study

Purpose of the study. To give a qualitative and quantitative assessment of different types of tumor microvessels depending on the histological type of cervical cancer (CC).

Material and methods

Material and methods. The tumor samples from 76 patients with CC stage I–IIA who received the surgical treatment on the first phase were the object of study. The immunohistochemical study was performed using antibodies to CD34 and podoplanin. Statistical analysis of the data was performed using the Statistica 6.0 software.

Results

Results. In CC, the following types of tumor microvessels have been identified: normal microvessels, dilated capillaries (DCs) with a normal endothelial lining, DCs with a weak expression of CD34, DCs of contact type, capillaries in the solid component of tumor, the atypical dilated capillaries (ADCs), the structures with partial endothelial lining and the lymphatic capillaries in lymphoid and polymorphic cell infiltrates. It was found that DCs with a mild expression of CD34 (p=0.0005), DCs of contact type (p=0.000005), and capillaries in the solid component of tumor (p=0.006) were correlated with the prognosis in early CC. The detection frequency of this tumor microvessels was lower in more prognostically favorable squamous non-keratinizing CC, while in the prognostically unfavorable squamous cell keratinizing CC and adenocarcinoma of the cervix uteri, the higher DCs values with a mild expression of CD34 (р=0,004 and р=0.005, respectively) and DC of contact type (p=0.02 and p=0.14, respectively) were typical.

Conclusion

Conclusion. We consider it is expedient to further study the relationship of different types of tumor microvessels with clinical and morphological characteristics and prognosis of cervical cancer.

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The authors declare that there are no conflicts of interest present.