Molecular genetic changes in kidney tissue in patients with COVID-19
https://doi.org/10.17709/2410-1893-2021-8-3-4
Abstract
Purpose of the study. Assessment of the molecular genetic profile of cytological processes in the kidney tissue of patients with COVID‑19.
Material and methods. Kidney fragments from patients with confirmed COVID‑19 (n = 96) were studied by real-time polymerase chain reaction to determine the expression of SARS-CoV‑2 viral RNA and genes encoding protein complexes: ACE‑2 and Furin.
Results. In patients affected by COVID‑19, the presence of coronavirus genetic material in kidney tissue was recorded, as well as increased expression of ACE‑2 (7.49 ± 0.27, p < 0.01) and Furin 2.0 times (8.59 ± 0.65, p < 0.01,) compared with the control group (3.9 ± 0.48, p < 0.01 and 4.2 ± 0.8, p < 0.01, respectively), which creates favorable conditions for the invasion of SARS-CoV‑2.
Conclusion. According to the results of RT-PCR for SARS-CoV‑2, assessment of the expression of ACE‑2 and Furin, it is possible with a high degree of probability to assert about the viral load and the vulnerability of the kidneys, since these proteins are informative markers of viral damage. Elderly people with increased vulnerability to SARS-CoV‑2.
About the Authors
G. A. Demyashkin
I.M.Sechenov First Moscow State Medical University (Sechenov University); National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation; . A.F.Tsyb Medical Radiological Research Center – Branch of the National Medical Research Radiological Center
Russian Federation
Competing Interests:
Russian Federation
Grigory A. Demyashkin – Cand. Sci. (Med.), MD, pathologist, Associate Professor at the Department of Pathological Anatomy named after Academician A.I.Strukov; leading researcher at the Department of Radiation Pathomorphology, SPIN: 5157-0177, AuthorID: 645433, Scopus Author ID: 57200415197
8/2 Trubetskaya str., Moscow 119991; 4 Koroleva str., Obninsk 249036; 4 Korolev str., Obninsk 249036
Competing Interests:
Аuthors report no conflict of interest
A. M. Mingazov
I.M.Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Competing Interests:
Russian Federation
Artemy M. Mingazov – Student at the N.V.Sklifosovsky Institute of Clinical Medicine
8/2 Trubetskaya str., Moscow 119991
Competing Interests:
Аuthors report no conflict of interest
E. A. Kaprina
I.M.Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Competing Interests:
Russian Federation
Elizaveta A. Kaprina – Student at the N.V.Sklifosovsky Institute of Clinical Medicine
8/2 Trubetskaya str., Moscow 119991
Competing Interests:
Аuthors report no conflict of interest
V. I. Shchekin
I.M.Sechenov First Moscow State Medical University (Sechenov University)
Russian Federation
Competing Interests:
Russian Federation
Vladimir I. Shchekin – Student at the N.V.Sklifosovsky Institute of Clinical Medicine, SPIN: 3664-8044, AuthorID: 1109647
8/2 Trubetskaya str., Moscow 119991
Competing Interests:
Аuthors report no conflict of interest
P. V. Shegay
National Medical Research Radiological Centre of the Ministry of Health of the Russian Federation
Russian Federation
Competing Interests:
Russian Federation
Petr V. Shegay – Cand. Sci. (Med.), Oncologist, Head of the Center for Innovative Radiological and Regenerative Technologies, SPIN: 6849-3221, AuthorID: 708894
4 Koroleva str., Obninsk 249036
Competing Interests:
Аuthors report no conflict of interest
References
1. Puelles VG, Lütgehetmann M, Lindenmeyer MT, Sperhake JP, Wong MN, Allweiss L, et al. Multiorgan and Renal Tropism of SARS-CoV-2. N Engl J Med. 2020 Aug 6;383(6):590–592. https://doi.org/10.1056/NEJMc2011400
2. Clarke NE, Turner AJ. Angiotensin-converting enzyme 2: the first decade. Int J Hypertens. 2012;2012:307315. https://doi.org/10.1155/2012/307315
3. Walls AC, Park Y-J, Tortorici MA, Wall A, McGuire AT, Veesler D. Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein. Cell. 2020 Apr 16;181(2):281-292.e6. https://doi.org/10.1016/j.cell.2020.02.058
4. Bradley BT, Maioli H, Johnston R, Chaudhry I, Fink SL, Xu H, et al. Histopathology and ultrastructural findings of fatal COVID-19 infections in Washington State: a case series. Lancet. 2020 Aug 1;396(10247):320–332. https://doi.org/10.1016/S0140-6736(20)31305-2
5. Kogan EA, Berezovsky YuS, Protsenko DD, Bagdasaryan TR, Gretsov EM, Demura SA, et al. Pathological Anatomy of Infection Caused by SARS-CoV-2. Russian Journal of Forensic Medicine. 2020;6(2):8–30. (In Russian). https://doi.org/10.19048/2411-8729-2020-6-2-8-30
6. Al Nemer A. Histopathologic and Autopsy Findings in Patients Diagnosed With Coronavirus Disease 2019 (COVID-19): What We Know So Far Based on Correlation With Clinical, Morphologic and Pathobiological Aspects. Adv Anat Pathol. 2020 Nov;27(6):363– 370. https://doi.org/10.1097/pap.0000000000000276
7. Hoffmann M, Kleine-Weber H, Schroeder S, Krüger N, Herrler T, Erichsen S, et al. SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell. 2020 Apr 16;181(2):271-280.e8. https://doi.org/10.1016/j.cell.2020.02.052
8. Zhou L, Niu Z, Jiang X, Zhang Z, Zheng Y, Wang Z, et al. SARSCoV-2 Targets by the pscRNA Profiling of ACE2, TMPRSS2 and Furin Proteases. iScience. 2020 Nov 20;23(11):101744. https://doi.org/10.1016/j.isci.2020.101744
9. Xiao L, Sakagami H, Miwa N. ACE2: The key Molecule for Understanding the Pathophysiology of Severe and Critical Conditions of COVID-19: Demon or Angel? Viruses. 2020 Apr 28;12(5):E491. https://doi.org/10.3390/v12050491
10. Xia S, Lan Q, Su S, Wang X, Xu W, Liu Z, et al. The role of furin cleavage site in SARS-CoV-2 spike protein-mediated membrane fusion in the presence or absence of trypsin. Signal Transduct Target Ther. 2020 Jun 12;5(1):92. https://doi.org/10.1038/s41392-020-0184-0
11. Borgmann H, Struck JP, Mattigk A, Wenzel M, Pilatz A, KranzJ, et al. Increased Severe Adverse Outcomes and Decreased Emergency Room Visits for Pyelonephritis: First Report of Collateral Damage during COVID-19 Pandemic in Urology. Urol Int. 2021;105(3–4):199–205. https://doi.org/10.1159/000513458
Supplementary files
Review
For citations:
Demyashkin G.A., Mingazov A.M., Kaprina E.A., Shchekin V.I., Shegay P.V. Molecular genetic changes in kidney tissue in patients with COVID-19. Research and Practical Medicine Journal. 2021;8(3):45-51. (In Russ.) https://doi.org/10.17709/2410-1893-2021-8-3-4
Views:
806
Email this article 