Role of hypothalamic-pituitary-adrenal axis and catecholamine factors in independent and primary multiple types of tumor growth
https://doi.org/10.17709/2410-1893-2022-9-4-5
Abstract
Purpose of the study. To study the levels of adrenal axis factors in the hypothalamus, adrenal glands, blood serum of mice and catecholamines in the adrenal glands during the independent growth of B16 melanoma and Lewis lung carcinoma (LLC) and their combined growth in female mice, and in males – with independent growth of B16 and combined growth of B16 and LLC.
Materials and methods. Male and female BALB/c Nude mice were divided into groups, n = 7 each: group 1 involved intact animals, group 2 involved mice with B16/F10 melanoma, group 3 – mice with LLC, group 4 – synchronous growth of melanoma and LLC. Levels of corticotropin releasing, noradrenaline and dopamine were determined in homogenates of the hypothalamus and adrenal glands and in the blood serum of all animals by ELISA, and levels of 17‑hydroxyprogesterone (17-OHP), dehydroepiandrosterone sulfate (DHEA-S) and cortisol were determined by RIA. Statistical processing of results was performed using the Statistica 10.0 program.
Results. All tumor-bearing females showed elevated corticotropin releasing in the hypothalamus together with an increase of all stress-characterizing parameters: cortisol, the cortisol/DHEA-S ratio, and noradrenaline. However, an increase in serum levels of cortisol was blocked by high levels of DHEA-S, and as a result, the cortisol/DHEA-S ratio was either within the normal range (B16 melanoma and B16+LLC combination) or reduced (LLC). Levels of corticotropin releasing in the hypothalamus of tumor-bearing males decreased, together with opposite changes in stress-characterizing parameters in the adrenal glands: cortisol increased, the cortisol/DHEA-S ratio did not differ significantly from the control values, and noradrenaline decreased. An increase in serum levels of cortisol was not blocked by high levels of DHEA-S, and as a result, the cortisol/DHEA-S ratio was sharply elevated in B16 melanoma and B16+LLC combination.
Conclusion. At independent and primary multiple types of tumor growth, the sex-specific features of the functioning of the adrenal axis at the central and peripheral levels are observed, which determines a more pronounced stressful state of the body with B16+LLC combination growth, realized by various mechanisms.
About the Authors
E. M. FrantsiyantsRussian Federation
Elena M. Frantsiyants – Dr. Sci. (Biol.), professor, general director’s substitute for science
Rostov-on-Don
SPIN: 9427-9928,
AuthorID: 462868,
ResearcherID: Y-1491-2018,
Scopus Author ID: 55890047700
V. A. Bandovkina
Russian Federation
Valeriya A. Bandovkina – Dr. Sci. (Biol.), senior researcher at the laboratory for the study of pathogenesis of malignant tumors
Rostov-on-Don
SPIN: 8806-2641,
AuthorID: 696989,
ResearcherID: AAG-8708-2019,
Scopus Author ID: 57194276288
I. V. Kaplieva
Russian Federation
Irina V. Kaplieva – Dr. Sci. (Med.), senior researcher at the laboratory for the study of pathogenesis of malignant tumors
Rostov-on-Don
SPIN: 5047-1541,
AuthorID: 734116,
ResearcherID: ААЕ-3540-2019,
Scopus Author ID: 23994000800
E. I. Surikova
Russian Federation
Ekaterina I. Surikova – Cand. Sci. (Biol.), senior research fellow at the laboratory for the study of the pathogenesis of malignant tumors
63 14 line str., Rostov-on-Don 344037
SPIN: 2401-4115,
AuthorID: 301537,
ResearcherID: AAG-8748-2019,
Scopus Author ID: 6507092816
Yu. A. Pogorelova
Russian Federation
Yulia A. Pogorelova – Cand. Sci. (Biol.), senior research fellow at laboratory of malignant tumor pathogenesis study
Rostov-on-Don
SPIN: 2168-8737,
AuthorID: 558241,
ResearcherID: AAE-4168-2022,
Scopus Author ID: 37026863400
N. D. Cheryarina
Russian Federation
Natalia D. Cheryarina – laboratory assistant at the laboratory for the study of the pathogenesis of malignant tumors
Rostov-on-Don
SPIN: 2189-3404,
AuthorID: 558243,
Scopus Author ID: 56204439400
L. K. Trepitaki
Russian Federation
Lidija K. Trepitaki – Cand. Sci. (Biol.), researcher assistant at the laboratory for the study of pathogenesis of malignant tumors
Rostov-on-Don
SPIN: 2052-1248,
AuthorID: 734359,
ResearcherID: AAG-9218-2019,
Scopus Author ID: 55357624700
I. V. Neskubina
Russian Federation
Irina V. Neskubina – Cand. Sci. (Biol.), senior research fellow at the laboratory for the study of the pathogenesis of malignant tumors
Rostov-on-Don
SPIN: 3581-8531,
AuthorID: 794688,
ResearcherID: AAG-8731-2019,
Scopus Author ID: 6507509066
A. A. Vereskunova
Russian Federation
Alexandra A. Vereskunova – student
Rostov-on-Don
I. M. Kotieva
Russian Federation
Inga M. Kotieva – D.Sci. (Med.), senior researcher at the laboratory of malignant tumor pathogenesis study
Rostov-on-Don
SPIN: 3478-5811,
AuthorID: 637665,
ResearcherID: AAJ-8320-2021,
Scopus Author ID: 56300093600
K. A. Shumarin
Russian Federation
Konstantin A. Shumarin – PhD student
Rostov-on-Don
SPIN: 5042-4897,
AuthorID: 1090463,
ResearcherID: AAК-1213-2021
A. I. Shikhlyarova
Russian Federation
Alla I. Shikhlyarova – Dr. Sci. (Biol.), professor, senior researcher, laboratory of study of malignant tumor pathogenesis
Rostov-on-Don
SPIN: 6271-0717,
AuthorID: 482103,
ResearcherID: Y-6275-2018,
AuthorID: 482103,
Scopus Author ID: 6507723229
I. V. Goroshinskaya
Russian Federation
Irina A. Goroshinskaya – Dr. Sci. (Biol.), professor, senior research fellow at the laboratory for the study of the pathogenesis of malignant tumors
Rostov-on-Don
SPIN: 9070-4855,
AuthorID: 79968,
ResearcherID: Y-2277-2018,
Scopus Author ID: 6602191458
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Supplementary files
Review
For citations:
Frantsiyants E.M., Bandovkina V.A., Kaplieva I.V., Surikova E.I., Pogorelova Yu.A., Cheryarina N.D., Trepitaki L.K., Neskubina I.V., Vereskunova A.A., Kotieva I.M., Shumarin K.A., Shikhlyarova A.I., Goroshinskaya I.V. Role of hypothalamic-pituitary-adrenal axis and catecholamine factors in independent and primary multiple types of tumor growth. Research and Practical Medicine Journal. 2022;9(4):52-62. (In Russ.) https://doi.org/10.17709/2410-1893-2022-9-4-5