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Role of hypothalamic-pituitary-adrenal axis and catecholamine factors in independent and primary multiple types of tumor growth

https://doi.org/10.17709/2410-1893-2022-9-4-5

Abstract

Purpose of the study. To study the levels of adrenal axis factors in the hypothalamus, adrenal glands, blood serum of mice and catecholamines in the adrenal glands during the independent growth of B16 melanoma and Lewis lung carcinoma (LLC) and their combined growth in female mice, and in males – with independent growth of B16 and combined growth of B16 and LLC.

Materials and methods. Male and female BALB/c Nude mice were divided into groups, n = 7 each: group 1 involved intact animals, group 2 involved mice with B16/F10 melanoma, group 3 – mice with LLC, group 4 – synchronous growth of melanoma and LLC. Levels of corticotropin releasing, noradrenaline and dopamine were determined in homogenates of the hypothalamus and adrenal glands and in the blood serum of all animals by ELISA, and levels of 17‑hydroxyprogesterone (17-OHP), dehydroepiandrosterone sulfate (DHEA-S) and cortisol were determined by RIA. Statistical processing of results was performed using the Statistica 10.0 program.

Results. All tumor-bearing females showed elevated corticotropin releasing in the hypothalamus together with an increase of all stress-characterizing parameters: cortisol, the cortisol/DHEA-S ratio, and noradrenaline. However, an increase in serum levels of cortisol was blocked by high levels of DHEA-S, and as a result, the cortisol/DHEA-S ratio was either within the normal range (B16 melanoma and B16+LLC combination) or reduced (LLC). Levels of corticotropin releasing in the hypothalamus of tumor-bearing males decreased, together with opposite changes in stress-characterizing parameters in the adrenal glands: cortisol increased, the cortisol/DHEA-S ratio did not differ significantly from the control values, and noradrenaline decreased. An increase in serum levels of cortisol was not blocked by high levels of DHEA-S, and as a result, the cortisol/DHEA-S ratio was sharply elevated in B16 melanoma and B16+LLC combination.

Conclusion. At independent and primary multiple types of tumor growth, the sex-specific features of the functioning of the adrenal axis at the central and peripheral levels are observed, which determines a more pronounced stressful state of the body with B16+LLC combination growth, realized by various mechanisms.

About the Authors

E. M. Frantsiyants
National Medical Research Centre for Oncology
Russian Federation

Elena M. Frantsiyants – Dr. Sci. (Biol.), professor, general director’s substitute for science

Rostov-on-Don

SPIN: 9427-9928,

AuthorID: 462868,

ResearcherID: Y-1491-2018,

Scopus Author ID: 55890047700



V. A. Bandovkina
National Medical Research Centre for Oncology
Russian Federation

Valeriya A. Bandovkina – Dr. Sci. (Biol.), senior researcher at the laboratory for the study of pathogenesis of malignant tumors

Rostov-on-Don

SPIN: 8806-2641,

AuthorID: 696989,

ResearcherID: AAG-8708-2019,

Scopus Author ID: 57194276288



I. V. Kaplieva
National Medical Research Centre for Oncology
Russian Federation

Irina V. Kaplieva – Dr. Sci. (Med.), senior researcher at the laboratory for the study of pathogenesis of malignant tumors

Rostov-on-Don

SPIN: 5047-1541,

AuthorID: 734116,

ResearcherID: ААЕ-3540-2019,

Scopus Author ID: 23994000800

 



E. I. Surikova
National Medical Research Centre for Oncology
Russian Federation

Ekaterina I. Surikova – Cand. Sci. (Biol.), senior research fellow at the laboratory for the study of the pathogenesis of malignant tumors

63 14 line str., Rostov-on-Don 344037

SPIN: 2401-4115,

AuthorID: 301537,

ResearcherID: AAG-8748-2019,

Scopus Author ID: 6507092816 



Yu. A. Pogorelova
National Medical Research Centre for Oncology
Russian Federation

Yulia A. Pogorelova – Cand. Sci. (Biol.), senior research fellow at laboratory of malignant tumor pathogenesis study

Rostov-on-Don

SPIN: 2168-8737,

AuthorID: 558241,

ResearcherID: AAE-4168-2022,

Scopus Author ID: 37026863400 



N. D. Cheryarina
National Medical Research Centre for Oncology
Russian Federation

Natalia D. Cheryarina – laboratory assistant at the laboratory for the study of the pathogenesis of malignant tumors

Rostov-on-Don

SPIN: 2189-3404,

AuthorID: 558243,

Scopus Author ID: 56204439400



L. K. Trepitaki
National Medical Research Centre for Oncology
Russian Federation

Lidija K. Trepitaki – Cand. Sci. (Biol.), researcher assistant at the laboratory for the study of pathogenesis of malignant tumors

Rostov-on-Don

SPIN: 2052-1248,

AuthorID: 734359,

ResearcherID: AAG-9218-2019,

Scopus Author ID: 55357624700



I. V. Neskubina
National Medical Research Centre for Oncology
Russian Federation

Irina V. Neskubina – Cand. Sci. (Biol.), senior research fellow at the laboratory for the study of the pathogenesis of malignant tumors

Rostov-on-Don

SPIN: 3581-8531,

AuthorID: 794688,

ResearcherID: AAG-8731-2019,

Scopus Author ID: 6507509066



A. A. Vereskunova
Rostov State Medical University
Russian Federation

Alexandra A. Vereskunova – student

Rostov-on-Don



I. M. Kotieva
National Medical Research Centre for Oncology
Russian Federation

Inga M. Kotieva – D.Sci. (Med.), senior researcher at the laboratory of malignant tumor pathogenesis study

Rostov-on-Don

SPIN: 3478-5811,

AuthorID: 637665,

ResearcherID: AAJ-8320-2021,

Scopus Author ID: 56300093600



K. A. Shumarin
National Medical Research Centre for Oncology
Russian Federation

Konstantin A. Shumarin – PhD student

Rostov-on-Don

SPIN: 5042-4897,

AuthorID: 1090463,

ResearcherID: AAК-1213-2021



A. I. Shikhlyarova
National Medical Research Centre for Oncology
Russian Federation

Alla I. Shikhlyarova – Dr. Sci. (Biol.), professor, senior researcher, laboratory of study of malignant tumor pathogenesis

Rostov-on-Don

SPIN: 6271-0717,

AuthorID: 482103,

ResearcherID: Y-6275-2018,

AuthorID: 482103,

Scopus Author ID: 6507723229



I. V. Goroshinskaya
National Medical Research Centre for Oncology
Russian Federation

Irina A. Goroshinskaya – Dr. Sci. (Biol.), professor, senior research fellow at the laboratory for the study of the pathogenesis of malignant tumors

Rostov-on-Don

SPIN: 9070-4855,

AuthorID: 79968,

ResearcherID: Y-2277-2018,

Scopus Author ID: 6602191458



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Frantsiyants E.M., Bandovkina V.A., Kaplieva I.V., Surikova E.I., Pogorelova Yu.A., Cheryarina N.D., Trepitaki L.K., Neskubina I.V., Vereskunova A.A., Kotieva I.M., Shumarin K.A., Shikhlyarova A.I., Goroshinskaya I.V. Role of hypothalamic-pituitary-adrenal axis and catecholamine factors in independent and primary multiple types of tumor growth. Research and Practical Medicine Journal. 2022;9(4):52-62. (In Russ.) https://doi.org/10.17709/2410-1893-2022-9-4-5

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ISSN 2410-1893 (Online)